Journal: Plant Mol Biol Rep (2013) 31:925 – 935
Author: Guangsheng Yuan, Zhiming Zhang , Kui Xiang, Yaou Shen, Juan Du, Haijian Lin, Li Liu, Maojun Zhao, Guangtang Pan
Abstract:
Ear rot, caused by Fusarium verticillioides (FV), is a destructive disease of maize as it reduces grain yield and increases risks of mycotoxin production, thus endangering live-stock. To identify genes differentially expressed during FV infection, four cDNA libraries were constructed for suppression subtractive hybridization using RNA isolated from bracts of an FV-resistant inbred maize line, Bt-1 , as well as an FV-susceptible maize inbred line, Ye478. A total of 145 clones were obtained following reverse dot-blot hybridization, and these were sequenced from these libraries. Similarity analysis revealed that of these genes , 93 were unique, including 68 of known function, 24 of unknown function, and a single novel gene . Most genes of known function were predominantly involved in plant defense such as cell defense, transcription regulation , signal transduction, and metabolism. Expression profiles of eight representative genes, using semiquantitative reverse transcription- polymerase chain reaction, confirmed that differential gene regulation during FV infection was involved. These findings suggested that these differentially expressed genes might be involved in FV defense responses in maize.
Link: http://link.springer.com/content/pdf/10.1007%2Fs11105-013-0567-2.pdf